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產品名稱：重組小鼠制瘤素M.蛋白
產品型號：rMuOSM
產品廠商：KALANG
產品文檔：

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簡單介紹：

重組小鼠制瘤素M.蛋白與其它公司提供的重組蛋白不同，rMuOSM蛋白產品為采用CFS的無細胞麥胚蛋白合成系統表達出來的重組蛋白，可表達出對細胞有毒性、易被蛋白酶降解的蛋白；并獲得具有良好的可溶性，并有翻譯后修飾、從而部分具有功能的蛋白.同時獨有的全自動蛋白純化技術則簡便高效，將蛋白純化過程中對蛋白的損傷降低到*小程度.重組小鼠制瘤素M.蛋白（全長序列）產品可用于Western Blot驗證、抗體制備、蛋白檢測、ELISA等試驗中.

詳情介紹：

重組小鼠制瘤素M.蛋白

Species Murine

Accession P53347

GeneID 18413

Source Escherichia coli.

Molecular Weight 重組小鼠制瘤素M.蛋白Approximately 20.4 kDa, a single non-glycosylated polypeptide chain containing 181 amino acids.

Quantity 2μg/10μg/1000μg

AA Sequence NRGCSNSSSQ LLSQLQNQAN LTGNTESLLE PYIRLQNLNT PDLRAACTQH SVAFPSEDTL RQLSKPHFLS TVYTTLDRVL YQLDALRQKF LKTPAFPKLD SARHNILGIR NNVFCMARLL NHSLEIPEPT QTDSGASRST TTPDVFNTKI GSCGFLWGYH RFMGSVGRVF REWDDGSTRS R

Purity > 96 % by SDS-PAGE and HPLC analyses.

Biological Activity 重組小鼠制瘤素M.蛋白Fully biologically active when compared to standard. The ED₅₀ as determined by a cell proliferation assay using murine NIH-3T3 cells is less than 1.0 ng/ml, corresponding to a specific activity of > 1.0 × 10⁶ IU/mg.

Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation Lyophilized from a 0.2 μm filtered concentrated solution in 2 × PBS, pH 7.4, 5 % trehalose.

Endotoxin Less than 1 EU/μg of rMuOSM as determined by LAL method.

Reconstitution 重組小鼠制瘤素M.蛋白We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

Storage This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.

重組小鼠制瘤素M.蛋白

SDS-PAGE

Reference 1. Tanaka M, Miyajima A. 2003. Rev Physiol Biochem Pharmacol. 149:39-52.
2. Mosley B, De Imus C, Friend D, et al. 1996. J Biol Chem. 271:32635-43.
3. Malik N, Kallestad JC, Gunderson NL, et al. 1989. Mol Cell Biol. 9:2847-53.
4. Brown TJ, Rowe JM, Liu JW, et al. 1991. J Immunol. 147:2175-80.

Background Oncostatin M (OSM) is a multifunctional cytokine that belongs to the Interleukin-6 subfamily. Among the family members, OSM is most closely related to leukemia inhibitory factor (LIF) and it in fact utilizes the LIF receptor in addition to its specific receptor in the human. A biologically active OSM receptor has been previously described that consists of a heterodimer of leukemia inhibitory factor receptor (LIFR) and gp130. OSM is synthesized by stimulated T-cells and monocytes. Furthermore, the effects of OSM on endothelial cells suggest a pro-inflammatory role for OSM and endothelial cells possess a large number of OSM receptors. Recombinant murine OSM contains 181 amino acids and has a molecular mass of 20.4 kDa. It has approximately 48 % and 72 % amino acid sequence identity with human and rat OSM.

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Species	Murine
Accession	P53347
GeneID	18413
Source	Escherichia coli.
Molecular Weight	重組小鼠制瘤素M.蛋白Approximately 20.4 kDa, a single non-glycosylated polypeptide chain containing 181 amino acids.
Quantity	2μg/10μg/1000μg
AA Sequence	NRGCSNSSSQ LLSQLQNQAN LTGNTESLLE PYIRLQNLNT PDLRAACTQH SVAFPSEDTL RQLSKPHFLS TVYTTLDRVL YQLDALRQKF LKTPAFPKLD SARHNILGIR NNVFCMARLL NHSLEIPEPT QTDSGASRST TTPDVFNTKI GSCGFLWGYH RFMGSVGRVF REWDDGSTRS R
Purity	> 96 % by SDS-PAGE and HPLC analyses.
Biological Activity	重組小鼠制瘤素M.蛋白Fully biologically active when compared to standard. The ED₅₀ as determined by a cell proliferation assay using murine NIH-3T3 cells is less than 1.0 ng/ml, corresponding to a specific activity of > 1.0 × 10⁶ IU/mg.
Physical Appearance	Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation	Lyophilized from a 0.2 μm filtered concentrated solution in 2 × PBS, pH 7.4, 5 % trehalose.
Endotoxin	Less than 1 EU/μg of rMuOSM as determined by LAL method.
Reconstitution	重組小鼠制瘤素M.蛋白We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Storage	This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
重組小鼠制瘤素M.蛋白
SDS-PAGE
Reference	1. Tanaka M, Miyajima A. 2003. Rev Physiol Biochem Pharmacol. 149:39-52. 2. Mosley B, De Imus C, Friend D, et al. 1996. J Biol Chem. 271:32635-43. 3. Malik N, Kallestad JC, Gunderson NL, et al. 1989. Mol Cell Biol. 9:2847-53. 4. Brown TJ, Rowe JM, Liu JW, et al. 1991. J Immunol. 147:2175-80.
Background	Oncostatin M (OSM) is a multifunctional cytokine that belongs to the Interleukin-6 subfamily. Among the family members, OSM is most closely related to leukemia inhibitory factor (LIF) and it in fact utilizes the LIF receptor in addition to its specific receptor in the human. A biologically active OSM receptor has been previously described that consists of a heterodimer of leukemia inhibitory factor receptor (LIFR) and gp130. OSM is synthesized by stimulated T-cells and monocytes. Furthermore, the effects of OSM on endothelial cells suggest a pro-inflammatory role for OSM and endothelial cells possess a large number of OSM receptors. Recombinant murine OSM contains 181 amino acids and has a molecular mass of 20.4 kDa. It has approximately 48 % and 72 % amino acid sequence identity with human and rat OSM.

產品名稱：重組小鼠制瘤素M.蛋白